| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Team Challenger FC - North Team Challenger FC B07 Black |
D310-052B3U-0008 | Matthew Kutner |
| A2 | Appleseed Premier Appleseed Premier Taylor |
D354-01XB3U-0002 | Ashton Taylor |
| A3 | Springfield FC SFC B2007 Black |
D442-012B3U-0002 | Dan Schlieman |
| A4 | Cleveland United Soccer Club Canton United Force 2007 Eagles |
D251-012B3U-0006 | William Haymaker |
| B1 | Pennine United Pennine Utd B07 White |
D2AJ-012B3U-0004 | Wayne Jentas |
| B2 | Greater Toledo Futbol Club Impact United 07 Boys Grey |
D444-01AB3U-0019 | Sean Neidemire |
| B4 | Team Challenger FC - South Team Challenger FC South 07 |
D310-012B3U-0006 | Walter Senk |