| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Internationals Soccer Club Internationals 2009 Red |
D299-01AG1U-0003 | Mike Stoerkel |
| A2 | Concordia Soccer Club Concordia 09 Girls |
D334-01AG1U-0001 | John Stalzer |
| A3 | Team Challenger FC - South Team Challenger FC South 09 |
D310-012G1U-0001 | Colin Curtis |
| A4 | Pacesetter Soccer Club Pacesetter 2009 Girls ONPL |
D455-012G1U-0004 | Erika Kimple |
| B1 | Team Challenger FC - North Team Challenger FC G09 Black |
D310-052G1U-0002 | Chris Dore |
| B2 | CSA Impact United Cleveland Force 09G Pre-DA |
D250-012G1U-0002 | Everett Palache |
| B3 | Greater Toledo Futbol Club GTFC Impact 09 Premier |
D444-01AG1U-0002 | Michael Aubry |
| C1 | Pacesetter Soccer Club Pacesetter 2009 Girls Black |
D455-012G1U-0005 | Paolo Guembes |
| C2 | Cleveland Everest Futbol Club Cleveland FC 09G |
D295-01AG1U-0002 | Andrew Dickson |
| C3 | Internationals Soccer Club Internationals 2009 Blue |
D299-01AG1U-0004 | Dean Dishman |