| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Internationals Soccer Club Internationals 2008 Red |
D299-01AG2U-0005 | Mike Stoerkel |
| A2 | Greater Toledo Futbol Club GTFC Impact 08 White |
D444-01AG2U-0004 | Trish Miller |
| A3 | FCA Oregon FCA NWO 08 Orange |
D471-023G12-0001 | Matt Nagel |
| A4 | Pacesetter Soccer Club Pacesetter 2008 Girls Black |
D455-012G2U-0006 | Jake Kirchoff-Sleet |
| B1 | Greater Toledo Futbol Club GTFC Impact 08 Premier |
D444-01AG2U-0003 | Michael Aubry |
| B2 | Cleveland United Soccer Club Canton United Force 2008 Blue |
D251-012G2U-0001 | Joe Pavlek |
| B3 | Pacesetter Soccer Club Pacesetter 2008 Girls ONPL |
D455-012G2U-0007 | Erika Kimple |
| B4 | Team Challenger FC - South Team Challenger FC South 08 |
D310-012G2U-0002 | Colin Curtis |
| C1 | CSA Impact United Cleveland Force 08G Pre-DA |
D250-012G2U-0003 | Denzil Antonio |
| C2 | Team Challenger FC - North Team Challenger FC G08 Black |
D310-052G2U-0003 | Allison Cuiksa |
| C3 | Cleveland Everest Futbol Club Cleveland FC 08G |
D295-01AG2U-0003 | Anthony Catanzarite |
| C4 | Internationals Soccer Club Internationals 2008 Blue |
D299-01AG2U-0006 | Dean Dishman |