| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Upper 90 Futbol Club Upper 90 North FC - 05 Girls Red |
D361-011G15-0001 | Attila Csiszar |
| A2 | Greater Toledo Futbol Club GTFC Impact 05 White |
D444-01AG5U-0009 | Scott Reed |
| A3 | Pacesetter Soccer Club Pacesetter 2005 Girls Black |
D455-012G5U-0012 | Daniel Fisher |
| A4 | CSA Impact United Cleveland Force 05G Blue |
D250-012G5U-0006 | Reilee DuPratt |
| B1 | Cleveland United Soccer Club Canton United Force 2005 Blue |
D251-012G5U-0003 | n/a |
| B2 | Pacesetter Soccer Club Pacesetter 2005 Girls Red |
D455-012G5U-0013 | Samantha Tiongson |
| B3 | Greater Toledo Futbol Club GTFC Impact 05 Premier |
D444-01AG5U-0010 | Michael Aubry |